The StemoniX microBrain 3D platform is a homogenous 3D neural spheroid system in 96 and 384-well format. . 315 can't find zero order. . NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreThe SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. In this product video, see how the SpectraMax M5 quickly reads samples. 6% for the on-board optics (monochromators). Unlimited breadth of. The SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer is equipped with transmitted light (TL), green fluorescence (Ex/Em: 460/541), and red (625/713) fluorescence channels. . 3: The cuvette chamber. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. SpectraMax iD3 and iD5 Register or Sign In. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. ; SoftMax® Pro 7. SpectraMax® i3x 多功能读板机配置 SpectraMax® MiniMax™ 300 细胞计数 仪，具有 transmitted light (TL)，绿色 (Ex / Em: 460 / 541) 和红色 (Ex / Em: 625 / 713) 荧光通道。MiniMax 细胞计数仪的 SoftMax® Pro 软件可以用来获得各种细 胞的图像并进行分析。本说. Introduction. i3x. 2. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. As an aspirant Bioscience research with expertise in cell biology, immunology and bio instrumentation techniques, I bring a wealth of knowledge and expertise to the table. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. 58% for the detection module and 2. Description 1. D 1 1. Use the 3. All the readers were operated using SoftMax® Pro Software, which also has built-in data analysis and curve-fitting for automatic calculation of EC 50. SpectraMax® Microplate Readers and SoftMax® Pro Software. The M5 can read volumes down to 2uL with the uMax Low. Biochrom New EX Read 400 Microplate Reader. The dual auto injector module. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The soil suspension (600 µL) and the substrate (L-3,4-dihydroxyphenylalanine, DOPA, 150 uL, 25 mmol L -1 ), were mixed and then added to. Spectranet customer portal. SoftMax Pro User’s Manual v Contents Figures. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when. . Image analysis provides accurate data. Supplier: Molecular Devices. See Unpacking the SpectraMax Paradigm Instrument on page 1. Itemploysa. Keywords: htrf spectramax detection cartridge, spectramax i3, spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. ClonePix 2 Mammalian Colony Picker; QPix Microbial Colony Pickers; CloneSelect Imager; DispenCell Single-cell Dispenser; 3D. SpectraMax® Microplate Readers and SoftMax® Pro Software. Catalog excerpts. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. I am a person who can handle a range of laboratory and data management tasks with precision and accuracy. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. ZERO BIAS - scores, article reviews, protocol conditions and moreThe SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and. com The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. . Spectramax i3 manualSpectramax i3 manual Microplate spectramax i3x molecular biocompareSpectramax i3. ; The top 3 importers of Spectramax are India with 365. SpectraMax Quant dsDNA Assay Kits. Download SoftMax® Pro 7. . 312 gain calibration failed. D 1 1. Transcreener® HTS is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes—many of which catalyze the covalent regulatory. no. Specifically, we present neurite. Modularity does provide some measure of forward vision into upcoming or future experimental needs. Expand your lab’s capabilities to include flash applications such as dual luciferase and. Background subtraction was not necessary. The information below applies to a standard 96 well plate, set to a linear shake mode. i3x Specifications. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. 0 mm hex key to remove the six blue retaining screws that secure the transport bracket (B) to the instrument. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Detection cartridge modularity allows for easy addition of new read modes within minutes without. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. ImageXpress Confocal HT. using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. Amplitude : 1. For each kit, the reader was able to detect the lowest dilution as positive. Expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays with the SpectraMax® i3x injector module with SmartInject® Technology ensuring equal mixing across the plate for high-precision experiments. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors; It allows you to explore cellular pathways and protein activation and expression in one system;In this application note, we describe how the SpectraMax ® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. Figure 1. The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. 313 reference gain check fail. 0. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). 3 STANDARDIZE TO A SINGLE ANALYSIS PLATFORMLearn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. . With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Structured Review. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. The SpectraMax i3x reader allows one to unravel the mysteries of science by exploring cellular pathways and protein. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. It allows you to explore cellular pathways and protein activation and expression in one system. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Description 1. Learn more about its features, specifications, and applications in this user guide. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. 22mm 384 FilterMax™F3&F5Multi-ModeMicroplateReaders,SpectraMax®i3x Multi-ModeDetectionPlatform,SpectraMax®iD3&iD5Multi-Mode MicroplateReaders,andSpectraMax®Paradigm®Multi-Mode MicroplateReader 5301193 18mm 96 SpectraMaxi3x,SpectraMaxiD3,SpectraMaxiD5,SpectraMaxM. 蛍光 発光 ELISA. Figure 4. For more sample throughput, the system canMicroplates were sealed to reduce evaporation (adhesive PCR plate seal; Thermo Fisher Scientific, AB0558) and placed in a microplate reader (Molecular Devices, SpectraMax i3x) for 8 h at 30 °C. . Bioz Stars score: 98/100, based on 1 PubMed citations. Description 1. Antigen Quantitation Protocol. #0200-7009) Contact our support team with your questions regarding Molecular Devices products and applications. each new assay. techsupport. Molecular Devices SpectraMax i3x Multi-Mode Microplate Platform. *On the SpectraMax i3x reader only. The SpectraMax i3x reader features a cooled photomultiplier tube (PMT) for low background noise in luminescence. The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells at a great price. Videos & Webinars. Expanded dynamic range Engineered for performance with spectral fusion illumination for increased sensitivity across the entire excitation range. 6% for the on-board optics (monochromators). The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Average 98 stars, based on 1 article reviews. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. . Whether you are looking for an intuitive, easy-to-use reader for. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. SpectraMax i3, i3x, and iD5 readers. 7 mm for all shake intensities. Multimode Readers Comparison Chart. 性能特点MD SpectraMax i3X 多功能荧光检测仪为采用光谱融合照明设计、测试功能模块化卡盒设计，具有细胞成像分析单元。. Prepare your iD series instrument for shipment. RIDASCREEN Gliadin competitive ELISA. Overview of Western Blot Cartridge. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 302 low ref light. 1. Sensitive TRF detection of two simple, mix-and-measure assay workflows. Data were analyzed with SoftMax ® Pro Software and graphed using a 4-parameter curve fit. com. Eliminate time-dependent substrate addition steps. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Eliminate time-dependent substrate addition steps. Bioz Stars score: 98/100, based on 1 PubMed citations. Expanded dynamic range The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube. which chemiluminescence was detected on a SpectraMax i3x reader (Molecular Devices) and analyzed us ing GraphPad. +1 800-635-5577. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Methods. 306 plate air cal. HeLa cells were plated at 15,000 cells per well in 100 µL. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic G q -coupled receptor. Description. . ü; Instrument Diagnostics Mode (for SpectraMax® i3x, SpectraMax® iD3, SpectraMax® iD5, SpectraMax® Paradigm)Absorbance was measured on the SpectraMax i3x Multi-Mode Platform at 475 nm and 660 nm after 1-4 hours of incubation. 303 unable to cal dark current. AlphaScreen. This technique allows for quality control and assurance when performing surface functionalization of nanoparticles. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. SpectraMax® i3x. Example: [Setup]. ，高内涵成像分析技巧——3D功能展示1-3D预览、共定位、测距，SpectraMax Mini 多功能微孔板读板机带你自信地迈出第一步！ ，多功能酶标仪 SpectraMax i3x 之动力学检测法的设置，iD5注射器卡盒操作视频 - 20201228_8，SpectraMax iD 系列触控屏 overview，【网络讲堂】高内涵. Shake intensity. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Curves were forced through x,y = 0. 5577 Web: Email: info@moldev. 012 fmol 0. 303 unable to cal dark current. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. 0´ 104 1. SpectraMax® L reader support; ü. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. Fluorescent Polarization: Solid black plates, as above. . SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析Spectramax i3x fluorescence plate reader to measure fluo-rescence resonance energy transfer (FRET) signals from cell lines expressing CFP-YFP FRET sensors for glucose and ATP, as these sensors give large changes in FRET ratio in response to appropriate conditions, and are expressed in cells at high levels. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,MDC instruments including: SpectraMax®, VersaMax®, VMax®, EMax®, Gemini, ThermoMax®, and FlexStation®. SpectraMax Paradigm Platform in conjunction with AlphaLISA provides a powerful tool to screen novel therapeutics in a pathologically relevant, endothelial cell-based model. 0. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析 We would like to show you a description here but the site won’t allow us. 더 많은. Reaction catalyzed by the firefly luciferase. 0´ 104 4. • SpectraMax i3x • Onboard luminescence • Detection cartridge (LUM 96) • SpectraMax iD5 • SpectraMax L • SpectraMax M5e • SpectraMax Mini. They include the SpectraMax® iD3 Multi-Mode Microplate Reader, SpectraMax® L Microplate Reader, SpectraMax® i3x Multi-Mode Microplate Reader, and SpectraMax® M5 Multi-Mode Microplate Reader. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. The SpectraMax i3x reader provides theThe SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyThe SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. Overview of Western Blot Cartridge. Description 1. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax i3x Multi-Mode microplatereader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging and Fast Kinetics with Injectors. Analysis. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Description 1. 0´ 104 6. to View Figures. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. ZERO BIAS - scores, article reviews, protocol conditions and more. Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Western blot assay 5 x 105 cells were seeded in 24-well plates in 500 μl growth medium and stimulated with novel compounds at 10μM. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringFluorescence at 365 nm excitation and 450 nm emission filters was determined based on a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA). SpectraMax Microplate Readers. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. com. Remove the transport bracket and store the retaining screws in the bag that held the 3. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. We would like to show you a description here but the site won’t allow us. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization options in the instrument settings in SoftMax Pro Software). The treatments were. 304 signal level saturation. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. 001 0. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. SeeThe SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles). For wavelengths in the UV range above 220 nm,. Molecular Devices ScanLater Western Blot Detection Platform. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. The SpectraMax i2X is used for cell migration, cell. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. g. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析We would like to show you a description here but the site won’t allow us. Flash-type luminescence with excellent sensitivity Expression measurement in as few as ten cells per well Improved accuracy of results by. ValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. The Curcumin binding to p53Y220C was also measured using the emission maxima of Curcumin at 495 nm after. 13 There are a fairly limited number ofSARS-CoV-2 variants of concern (VOCs) that increase transmission or disease severity or reduce diagnostic or vaccine efficacy continue to emerge across the world. Time-. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. Supplier: Molecular Devices. Instrument. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. 2 nM was calculated using SoftMax Pro Software. Summary of Contents for Molecular Devices SpectraMax M2. Examples: “Press carefully to engage,” “ Do not. The SpectraMax i3x reader provides theSoftMaxProSoftwareFormulaReferenceGuide 8 5049093A SummaryFormulas Summariesconsistoffourparts: ASummaryname(whichisusedtorefertotheSummaryinotherformulas). Downloads. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. FlexStation® 3 Multi-Mode Microplate Reader. Measured using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 0´ 105 dsD NA (p g/ wel ) R F UFluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). . Figure 1. . SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. We would like to show you a description here but the site won’t allow us. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. SpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. Bioz Stars score: 98/100, based on 1 PubMed citations. 313 reference gain check fail. Sufficient for 1000 reactions. BMG LABTECH VANTAstar. Description 1. 7 Figures from 1126 Publications. Find regional support. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. 99 to $1999. ValitaTiter Plus settingsContact our support team with your questions regarding Molecular Devices products and applications. In order to calculate a humification index (HIX), spectra were obtained with an excitation wavelength of 254 nm over the emissions waveband of 300–480 nm at increments of 5 nm (Ohno 2002. The calculated LOQ values were 0. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellEvery SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. The ELISA plates were read at an absorbance of 450 nm on a SpectraMax i3x Plate Reader using Softmax Pro 7. As shown in the results above, the DELFIA cell proliferation assay combined with the SpectraMax i3 reader gives researchers a very sensitive test for measuring cell proliferation. Microplate Readers SpectraMax. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. Published Dec 16, 2020 | Updated Oct 10, 2023. SpectraMax® i3x Multi-Mode Detection Platform; SpectraMax® i3 Multi-Mode Detection Platform; SpectraMax® ID3 Multimode Microplate reader; SpectraMax® ID5 Multimode Microplate reader; SpectraMax® Paradigm® Multi-Mode Microplate Reader; SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. The SpectraMax i3x Multi-Mode Microplate Reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. SpectraMax ® i3/i3x Multi-Mode Microplate Reader; SpectraMax Plus 384 Absorbance Microplate Reader; Methods. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Explore more about Optimized settings for Transcreener TR-FRET assay on SpectraMax iD5 and SpectraMax i3x readers. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. With optimized reagents, option to operate the. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. The SpectraMax i3x is a multi-mode microplate reader that measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. GloMax Discover System. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Cell viability는 세포막 Integrity 또는. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. Molecular Devices Software. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. . Molecular Devices Spectramax 340 Absorbance Plate Reader (1997) Molecular Devices SpectraMax i3x Multi-Mode Microplate Reader (2016) Vernier UV-VIS Spectrophotometer (5) Vernier VIS Spectrophotometer (30) Infrared Spectroscopy. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. 1 Microplate Data Acquisition & Analysis Software for Windows. Italic and boldface type are used for emphasis. Price from $9. The assay can accurately test the toxicology of compounds as shown in Figure 1. 1. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex. ü; SpectraMax® Mini reader support. SPECTRAMAX i3X, INJECTOR MODULE CARTRIDGE model number 0200-7029. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring Download SoftMax® Pro 7. Absorbance Fluorescence (top/bottom read) Luminescence (top read) Time-Resolved Fluorescence (top read). Prepare your i3/i3x instrument for shipment. SpectraMax i3x Register or Sign In. Explore a wealth of applications with the future-ready SpectraMax i3x. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Learn how you can perform flash-based applications, such as dual luciferase and ATP assays, with high precision and accuracy using this cartridge. 3 Figures from 612 Publications Promega. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. (SpectraMax i3/i3x) CatchPoint SimpleStep ELISA (SpectraMax Paradigm) SpectraDrop. Ribonuclease (RNase) R digestion assay For identifying the stabilization of circVRK1, 2 μg isolated RNA from HBMECs was digested by RNase R (3 U/μg; RNR07250, Geneseed, Guangzhou,. 304 signal level saturation. pH-Xtra Glycolysis Assay - Agilent. Unlimited breadth of. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideProducts : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. Note: Additional readers with similar performance are listed. Failed to load PDF file. ，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. 305 reference level saturation. Figure 4. Products : SpectraMax i3x reader, SoftMax Pro GxP Software, SpectraMax i3, SoftMax Pro Software, SpectraMax MiniMax cytometer When I attempt a read with my SpectraMax i3 or i3x reader using the injector cartridge, why do I see a plate height error, even though I have entered the correct plate height?. The chamber. 0. SpectraMax i3x Register or Sign In. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The CellTiter-Glo assay from Promega uses luciferase. The SpectraMax® i3x reader allows you to. Request a Quote for This Setup. SpectraMax i3Instrumenttovisuallyinspectyoursampleandtoruncell-basedassaysat cellularorwhole-cellresolution. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. It has a cooled PMT, a no-stain cell analysis algorithm, and a field-upgradeable imaging cytometer. In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. This process should take about 3 - 5 minutes. HTRF IP standard curve. 2. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . Price from $9. Products : SoftMax Pro GxP Software, SoftMax Pro Software. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. 9 x 10-15. Wokingham, Berkshire RG41 5TS United Kingdom. .